Effects of Propofol on Hepatoma Carcinoma HepG2 Cell Invasion
10.6039/j.issn.1001-0408.2016.07.11
- VernacularTitle:丙泊酚对肝癌HepG2细胞侵袭的影响
- Author:
Wenxing LI
;
Jincheng LI
- Publication Type:Journal Article
- Keywords:
Propofol;
Hepatoma carcinoma HepG2 cells;
Invasion;
MMP;
E-cadherin;
Snail;
NF-κB
- From:
China Pharmacy
2016;27(7):899-902
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of propofol on invasion of hepatoma carcinoma HepG2 cell. METHODS:MTT method was used to detect the viability of HepG2 cells which were cultured with 0(negative control),1,3 and 10 μg/ml propofol for 48 h. The ability of cell invasion was detect by Tranaswell method. The phosphorylation level of nuclear factor-κB p65(NF-κB p65) and the expression of matrix metalloproteinase 2 (MMP-2),MMP-9,E-cadhherin and Snail were detected by Western blot method. RESULTS:Compared with negative control,1,3,10 μg/ml propofol inhibited the cell viability and invasion,down-regu-lated the expression of Snail and the phosphorylation level of NF-κB p65,and up-regulated the expression of E-cadherin (P<0.01). 3,10μg/ml propofol down-regulated the expression of MMP-2 and MMP-9(P<0.01). Above effects depended on drug con-centration(P<0.01). CONCLUSIONS:Propofol can suppress HepG2 invasion,which might be related to the inhibition of NF-κB/Snail signal pathway.
