Effects of Thymalfasin for Injection on the Apoptosis of Humun Lung Cancer A549 Cells
10.6039/j.issn.1001-0408.2016.07.13
- VernacularTitle:注射用胸腺法新对人肺癌A549细胞凋亡的影响
- Author:
Zhaohui YANG
;
Ruixiang DUAN
;
Jing YANG
;
Dani XIONG
;
Jianmin LANG
- Publication Type:Journal Article
- Keywords:
Thymalfasin for injection;
Human lung cancer A549 cells;
Proliferation;
Apoptosis;
Akt signal pathway
- From:
China Pharmacy
2016;27(7):906-909
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Thymalfasin for injection on the apoptosis of human lung cancer A549 cells. METHODS:After treated with 0(blank control),25,50,100,200 and 400 mg/L Thymalfasin for injection for 24,48 and 72 h, the cell proliferation inhibitory rate was analyzed with MTT and calculated. After treated with 0(blank control),50 and 100 mg/L Thymalfasin for injection for 48 h,cell apoptosis was detected by flow cytometry,and the expression of Caspase-3,Bcl-2 and Bax and the phosphorylation level of Akt were deteced by Western blot. RESULTS:Compared with blank control group,proliferation in-hibitory rate of A549 cells increased after treated with Thymalfasin for injection,in concentration and time-dependent manner(P<0.05). The apoptotic rate of A549 cells increased after treated with Thymalfasin for injection 50,100 mg/L for 48 h (P<0.05). The expression of Caspase-3 increased while the Bcl-2/Bax and phosphorylation level of Akt decreased in A549 cells after treated with Thymalfasin for injection 100 mg/L (P<0.05). CONCLUSIONS:Thymalfasin for injection can inhibit the proliferation of A549 cells by activating Caspase-3,decreasing Bcl-2/Bax ratio,inhibiting Akt signal pathway and induce the apoptosis of A549 cells.
