Content Determination of Garlic Saponin in Allium sativum by Pre-column Derivatization-HPLC
10.6039/j.issn.1001-0408.2016.03.25
- VernacularTitle:柱前衍生化-HPLC法测定大蒜中甾体皂苷的含量
- Author:
Xingliang SONG
;
Shukun LIANG
;
Yimeng ZHAO
;
Peng WANG
- Publication Type:Journal Article
- Keywords:
Allium sativum;
Pre-column derivatization;
HPLC;
Steroidal saponins;
Sarsasapogenin
- From:
China Pharmacy
2016;27(3):361-364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of steroidal saponin in Allium sativum. METHODS:Pre-column derivatization of steroidal saponin was performed by using the derivatization agent of nitro-benzoic acid-chlorine. And HPLC was conducted to determine the content of steroidal saponin. The column was Shimadzu VP-ODS with mobile phase of aceto-nitrile-water mixed solution(80:20,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 254 nm,the column temper-ature was 25 ℃,and the injection volume of 20 μl. RESULTS:The linear rang of sarsasapogenin was 0-1.25 mg/ml(r=0.999 0);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 93.1%-96.8%(RSD=1.56%,n=6). CON-CLUSIONS:The method is simple,stable with good separation,and can be use for the content determination of steroidal saponin in A. sativum.
