Experimental study onα-Asarone-induced apoptosis in human esophageal carcinoma Eca-109 cell line I by regulating GADD153 and Smac mRNA expression levels
10.16571/j.cnki.1008-8199.2016.10.008
- VernacularTitle:α-细辛醚调控GADD153和 Smac mRNA表达诱导人食管癌细胞株Eca-109凋亡研究
- Author:
Yan ZHANG
;
Hang HE
;
Li WANG
;
Yanqin ZHU
- Publication Type:Journal Article
- Keywords:
α-Asarone;
Growth arrest and DNA damage-inducible gene 153;
Second mitochodria-derived activator of caspase
- From:
Journal of Medical Postgraduates
2016;29(10):1046-1049
- CountryChina
- Language:Chinese
-
Abstract:
Objective α?Asarone has the effect of relieving cough and asthma as well as sedative, hypnotic and anticonvul?sive function. Our study was designed to explore the effects of apoptosis induced by α? Asarone on human esophageal carcinoma Eca?109 cell line as well as the expression levels of GADD153 and Smac mRNA. Methods Human esophageal carcinoma Eca?109 cells were cultured in vitro, which were divided into blank control group, 5?FU group( 500μg/mL) andα?Asarone group of different dosages ( 25,50,100μg/mL) . After cultivation, MTT method and Annexin V?PI were used to measure cell proliferation rate and apoptosis rate. Expression levels of GADD153 and Smac protein and mRNA were detected by western blotting and real? time quantitative PCR. Results Compared with blank control group, the cell proliferation rates in other groups decreased significantly (P<0.01), and cell apoptosis rate increased significantly ( P<0.01) . Compared with blank control group, the expression levels of GADD153 and Smac pro?tein and mRNA in other groups increased significantly( P<0.05) . Conclusion α? Asarone can inhibit cell proliferation and induce the apoptosis of human esophageal carcinoma Eca?109 by regulating the expression levels of GADD153 and Smac gene.
