HPLC determination of oleanolic acid and ursolic acid in Qingdu tablet
10.3760/cma.j.issn.1673-4246.2016.10.018
- VernacularTitle:HPLC法测定清毒片中齐墩果酸和熊果酸含量
- Author:
Zhiheng LI
;
Yanfei LIN
;
Bijun HU
- Publication Type:Journal Article
- Keywords:
Qingdu tablet;
Hedyotis diffusa;
Oleanolic acid;
Ursolic acid;
High performance liquid chromatography;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2016;38(10):932-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for quality control of oleanolic acid and ursolic acid in Qingdu tablet. Methods The content of oleanolie acid and ursolic acid in Qingdu tablet were determined by high performance liquid chroma-tography(HPLC). The analytical column was kromasil-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.5% phosphoric acid solution (88:12). The UV detection wavelenghth was 210 nm. The flow rate was 1.0 ml/min. Results Oleanolic acid and ursolic acid showed a good linearity in range of 0.06-0.54μg (R=0.999 3) and 0.30-2.70μg (R=0.999 7). The average recovery rates of Oleanolic acid and ursolic acid were 100.48% and 102.21%, respectively. Conclusions This method is simple, rapid and accurate. It could be used for the quality control and quality study of the Qingdu tablet.
