Expression and identification of VEGF165 in bone marrow mesenchymal stem cells of rhesus
10.11958/20160444
- VernacularTitle:VEGF165在恒河猴骨髓间充质干细胞中的表达及鉴定
- Author:
Zaiyu GUO
;
Heliang ZHANG
;
Tao SHUI
;
Guozhe ZHANG
;
Weihua ZHAO
;
Qian CHEN
;
Yanwei HOU
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
vascular endothelial growth factor;
macaca mulatta;
transgenes;
mesenchymal stem cell transplantation;
green fluorescent proteins
- From:
Tianjin Medical Journal
2016;44(10):1209-1212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the transferred vascular endothelial growth factor (VEGF)165 gene expression in rhesus autologous bone marrow mesenchymal stem cells (MSCs), and to explore the functional viability of transgenic MSCs. Methods MSCs from rhesus bone were isolated by Ficoll, which were used to detect the phenotype. After the culturing, the expression vector pcDNA-eGFP-VEGF165 was transfected into bone marrow MSCs. Fluorescence microscope and flow cytometry were used to detect the enhanced green fluorescent protein (eGFP) expression. At the same time, the phenotype in transfected MSCs was also indentified. The VEGF165 expression level was detected by RT-PCR. Results The highly purified MSCs were collected successfully. The transfected MSCs and daughter cells showed expressions of eGFP and VEGF165, which also remained the characteristics of MSCs. Conclusion The VEGF165 gene that is transfected into MSCs can maintain characteristics of MSCs, and stably express foreign genes.
