The change of AChE related microRNAs derived from peripheral blood mononuclear cells in acute ischemic stroke
10.11958/20160518
- VernacularTitle:急性脑卒中患者外周血单个核细胞来源的AChE相关的microRNAs的表达变化
- Author:
Bin HAN
;
Xiaofeng MA
;
Chao ZHANG
- Publication Type:Journal Article
- Keywords:
stroke;
acetylcholinesterase;
microRNAs;
peripheral blood mononuclear cells;
cholinergic anti-inflammatory pathway
- From:
Tianjin Medical Journal
2016;44(10):1251-1254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of acetylcholinesterase (AChE) related microRNAs derived from peripheral blood mononuclear cells (PBMCs) in patients with stroke. Methods The microRNAs for targeting AChE mRNA were selected via prediction software and previous studies. PBMCs were extracted from venous blood samples of acute ischemic stroke patients (onset<24 h) and healthy controls. The expressions of microRNAs and AChE mRNA were quantified using real-time PCR (RT-PCR). The protein level of AChE was detected by Western blot assay. Results Thepredicted microRNAs included microRNA (miR)-24,-28,-124,-132,-182*,-194 and-484. The expression levels of miR-24,-124,-132 and-194 were significantly elevated in stroke patients compared with those of controls (P<0.05). There were no significant changes in expression levels of miR-28,-182*and-484. Additionally, the relative expression levels of intracellular AChE mRNA and protein decreased significantly in stroke patients (P<0.05). Conclusion MiRNAs can enhance cholinergic anti-inflammatory pathway by targeting AChE in patients with acute ischemic stroke.
