ATRA combined with IFN-α2a promotes iodine uptake of FTC-133 follicular thyroid carcinoma cells
10.16571/j.cnki.1008-8199.2016.09.004
- VernacularTitle:全反式维甲酸联合干扰素对滤泡状甲状腺癌细胞摄碘的促进作用
- Author:
Zhen ZHAO
;
Guohua SHEN
;
Huawei CAI
- Publication Type:Journal Article
- Keywords:
Thyroid neoplasm;
All-trans retinoic acid;
Interferon;
Iodine radioisotope
- From:
Journal of Medical Postgraduates
2016;29(9):910-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective Some drugs can effectively improve the uptake of 131 I in dedifferentiated thyroid cancer .This study was to investigate the effect of all-trans retinoic acid (ATRA) combined with interferon-α2a (IFN-α2a) on the iodine uptake and re-tention rate of FTC-133 follicular thyroid carcinoma cells . Methods FTC-133 cells were cultured in the presence of 2 μmol/L AT-RA and/or 1500 IU/mL IFN-α2a for 72 hours.The the cells were collected for measurement of the uptake and retention rate of Na 125 I. Results The Na125 I uptake of the FTC-133 cells was significantly increased after 72-hour incubation with 2μmol/L ATRA and 1500 IU/mL IFN-α2a (2423.1 ±237.5) as compared with those of the control (1279.5 ±102.8), ATRA (1438.2 ±149.6), and IFN-α2a groups (1355.3 ±198.4) (P<0.05).Statistically significant differences were observed in the retention rate of Na 125I in the FTC-133 cells at different time points in the each of the blank control , 2μmol/L ATRA, 1500 IU/mL IFN-α2a, and combination groups (P<0.01), but not in that of Na125I among these groups (P<0.05). Conclusion ATRA in combination with IFN-α2a can promote the Na125 I uptake but cannot improve the Na 125 I retention rate in FTC-133 cells.
