Self-assembly of plasma protein as a nanocarrier for lipophilic drugs
10.16571/j.cnki.1008-8199.2016.09.016
- VernacularTitle:血浆蛋白-脂溶性药物纳米载体体系的自组装
- Author:
Guangming GONG
;
Meihui CHEN
;
Yanli CAO
;
Shudong WANG
- Publication Type:Journal Article
- Keywords:
Plasma protein;
Assembly;
Hydrophobic drugs;
Nanocarrier system
- From:
Journal of Medical Postgraduates
2016;29(9):964-967
- CountryChina
- Language:Chinese
-
Abstract:
Objective Human serum albumin ( HSA) is a kind of plasma protein , which can transport various small molecu-lar substances to the body.A disulfide bond breaking strategy is used to fabricate HSA-curcumin nanoparticles(NPs-CCM) in order to improve the solubility and tumor targeting capability of curcumin . Methods NPs-CCM were fabricated with dithiothreitol as the re-ducing agent .The size and shape of the nanoparticles were observed with high resolution electron microscopy .We explored the change of hydrophobic region during the process of degeneration of albumin , the releasing behavior in the solution and the tumor targeting capa-bility in vivo and vitro of NPs-CCM. Results The diameter of NPs-CCM was about 150 nm.The determination of surface hydropho-bicity showed that ANS fluorescence intensity decreased gradually as the extension of degeneration time , at the time 0 min, 2 min, 5 min and 10 min, ANS relative fluorescence intensity was 5492(5432-5673), 2486(2319-2878), 1998(1904-2317), 1503 (1280-1584) respectively.The hydrophobic region of albumin gradually reduced during the degeneration time .The curcumin re-leased from NPs-CCM amounted to about 50% within 40 h, and the NPs-CCM can be absorbed by tumor cells and accumulation in tumor tissue. Conclusion Albumin can improve the solubility and tumor targeting capability of curcumin , and it has potential clinical application value.
