Pantoprazole sodium inhibits epithelial-mesenchymal transition and cisplatin resistance in lung cancer cells and underlying mechanism
10.3969/j.issn.1000-4718.2016.10.010
- VernacularTitle:质子泵抑制剂泮托拉唑钠抑制肺癌细胞上皮间质转化和顺铂耐药的机制研究
- Author:
Jia SONG
;
Jian WANG
;
Yu LI
;
Huizhen HU
;
Jie YAN
;
Lijun WU
;
Wei XU
;
Qingyong CHEN
- Publication Type:Journal Article
- Keywords:
Pantoprazole sodium;
Lung cancer;
Epithelial-mesenchymal transition;
Drug resistance
- From:
Chinese Journal of Pathophysiology
2016;32(10):1788-1798
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To explore the inhibitory effects of pantoprazole sodium on epithelial-mesenchymal transition and cisplatin resistance in lung cancer cells and the underlying mechanism .METHODS: Using MTT method, wound healing assay , Transwell experiment , Western blot , the differences of morphology , invasion ability , migration ability , drug sensitivity and protein expression between A 549/DDP cells and A549 cells were determined .The effect of pantoprazole so-dium on morphology , invasion ability , migration ability , drug sensitivity and protein expression in A 549/DDP cells were al-so observed.RESULTS: Compared with A549 cells, A549/DDP cells had higher invasion and migration abilities , and lower drug sensitivity , exhibited mesenchymal phenotype and activated c-Met/AKT/mTOR pathway .Pantoprazole sodium inhibited the abilities of invasion and migration , and reversed the mesenchymal phenotype , drug resistance and the c-Met/AKT/mTOR pathway activation in A549/DDP cells.Treatment with c-Met inhibitor SU11274, PI3K inhibitor LY294002 and mTOR inhibitor rapamycin had the same effects on A 549/DDP cells as that of pantoprazole sodium .CONCLUSION:Pantoprazole sodium inhibits invasion , migration, epithelial-mesenchymal transition and cisplatin resistance in lung cancer cells by down-regulating c-Met/AKT/mTOR pathways .
