Effects of transcription factor HOXB7 silencing on viability, invasion and migration of SGC-7901 cells
10.3969/j.issn.1000-4718.2016.10.014
- VernacularTitle:HOXB7基因沉默对人胃癌细胞活力、迁移和侵袭的影响
- Author:
Weili ZHANG
;
Chao CHEN
- Publication Type:Journal Article
- Keywords:
HOXB7 gene;
Gastric carcinoma;
Cell viability;
Cell invasion;
Cell migration
- From:
Chinese Journal of Pathophysiology
2016;32(10):1824-1829
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To explore the effects of transcription factor HOXB7 silencing on the viability , invasion and migration of SGC-7901 cells.METHODS:The siRNA was designed to specifically interfere the expression of HOXB7.To assess the silence efficiency of the siRNA , the expression of HOXB7 at mRNA and protein levels was detected by real-time PCR and Western blot in the SGC-7901 cells transfected with siRNA .The cell viability was measured by MTT assay .Cell cycle-related proteins such as cyclin D 1 and CDK4 were determined by Western blot .Transwell assay was performed to an-alyze the migration ability of the SGC-7901 cells.The mRNA and protein levels of the tumor invasion-and metastasis-relat-ed molecules, such as VEGF, PTEN and p-AKT, were also detected by real-time PCR and Western blot .RESULTS:The expression of HOXB7 at mRNA and protein levels was higher in the gastric carcinoma tissues than that in the normal gastric tissues.The expression of HOXB7 at mRNA and protein levels was knockdown by siRNA .After silencing of HOXB7 gene expression, the viability of the SGC-7901 cells decreased, the expression of cyclin D1, CDK4 and VEGF was down-regula-ted, and the phosphorylation level of AKT was also obviously decreased .CONCLUSION:HOXB7 effectively promotes the viability, invasion and migration of gastric carcinoma cells by up-regulating the expression of cyclinD 1, CDK4 and VEGF, and increasing the phosphorylation level of AKT to inhibit PTEN function .
