Effect of intranasal treatment with Toll-like receptor 9 ligand CpG oli-godeoxynucleotides on airway inflammation in mice with allergic com-bined airway disease
10.3969/j.issn.1000-4718.2016.10.015
- VernacularTitle:Toll样受体9配体CpG-ODN鼻内应用对变应性联合气道疾病炎性反应的影响
- Author:
Hongtao LI
;
Zhuanggui CHEN
;
Jin YE
;
Hui LIU
;
Xiaoling ZOU
;
Hailing YANG
;
Tiantuo ZHANG
- Publication Type:Journal Article
- Keywords:
Bronchial asthma;
Allergic rhinitis;
CpG oligodeoxynucleotides
- From:
Chinese Journal of Pathophysiology
2016;32(10):1830-1836
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the therapeutic effect of intranasal administration of CpG oligodeoxynucleotides (CpG-ODN), compared with intradermal administration, on lower airway inflammation in ovalbumin (OVA)-induced al-lergic combined airway disease (ACAD) mouse model.METHODS: Totally 30 female BALB/c mice aged from 6 to 8 weeks were randomly divided into control group , allergic rhinitis model group (AR group), ACAD group, ACAD intrana-sally treated with CpG-ODN group (CpG i.n.group) and ACAD intradermally treated with CpG-ODN group (CpG i.d. group).The mice were sensitized and challenged with OVA .Treatment with CpG-ODN was also performed during chal-lenge, either intranasally or intradermally .Immunologic variables and nasal symptom were studied .RESULTS:Compared with CpG i.d.group and ACAD group, the percentage of eosinophils from bronchoalveolar lavage fluid (BALF), the levels of Th2 cytokine production in BALF and supernatants of cultured splenic lymphocytes , OVA-specific IgE from blood , peri-bronchial inflammation score in the lung , and nasal symptoms were significantly reduced in CpG i .n.group.CONCLU-SION:Allergic rhinitis treated by CpG-ODN has a significant improvement on lower airway inflammation in ACAD mouse model;and it may be more effective when administrated intranasally than intradermally .
