Quantitative proteomics of CDC42 in HBx-mediated cellular transformation

VernacularTitle:CDC42在HBx介导肝细胞恶性转化中作用的定量蛋白质组学研究
Author: Yongru XU ; Yingzi QI ; Ping XU ; Xiangping LI ; Feng XU
Publication Type:Journal Article
Keywords: quantitative proteomics; cytoskeleton; hepatocellular carcinoma; CDC42; gene deletion
From: Military Medical Sciences 2016;40(9):697-702
CountryChina
Language:Chinese
Abstract: Objective To identify the mediators of CDC42 signaling pathway involved in hepatitis B virus X protein (HBx)-mediated cellular transformation.Methods The mass defect-based pseudo-isobaric dimethyl labeling method (pIDL)was used to detect the differentially expressed proteins with a deficiency of CDC42.Furthermore,we conducted a gene ontology (GO)of differentially expressed proteins.Results and Conclusion We totally qualified 3409 proteins and found 220 differentially expressed proteins.Palladin,formin-like 1 (FMNL1)and keratin-19,which were implicated in cytoskeleton organization,were down-regulated with the deficiency of CDC42.Our results have provided candidate genes and proteins that may play an important role in HBx-mediated cellular transformation.