Prokaryotic expression and purification of SET7 with GST-tag
10.7644/j.issn.1674-9960.2016.09.003
- VernacularTitle:组蛋白甲基转移酶SET7的原核表达及纯化鉴定
- Author:
Jie LIU
;
Zhengxiang FU
;
Yanan ZHANG
;
Yang JIANG
;
Lihua DING
;
Qinong YE
- Publication Type:Journal Article
- Keywords:
SET7 gene;
prokaryotic expression;
purification;
breast neoplasms
- From:
Military Medical Sciences
2016;40(9):707-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify and prokaryotically express the histone methyltransferase SET7.Methods The coding sequence of full length SET7 was amplified from breast library by PCR and cloned into the pGEX-KG vector.The correct recombinant plasmid was introduced into E.coli.The expressed protein was identified by SDS-PAGE.Results DNA sequencing indicated the SET7 was constructed.GST-SET7 fusion protein was identified by SDS-PAGE.Conclusion The prokaryotically expressed protein of GST-SET7 is obtained,which will falilitate further study of SET7 function.
