Mechanism of Escherichia coli injury under UV disinfection

VernacularTitle:紫外线对大肠杆菌的损伤机制研究
Author: Jing LI ; Daning WANG ; Hongmei QU ; Zhigang QIU ; Jing YIN ; Junwen LI ; Min JIN
Publication Type:Journal Article
Keywords: ultraviolet rays; disinfection; Escherichia coli; bacterial injury; spectrum analysis,Raman; microscopy,electron,transmission
From: Military Medical Sciences 2016;40(9):725-728
CountryChina
Language:Chinese
Abstract: Objective To study the inactivation kinetics and injury mechanism of Escherichia coli under UV disinfection in drinking water.Methods The inactivation kinetics of E.coli ATCC 25922 was determined by plate count methods in the UV disinfection experiment.The morphology,cell membrane permeabilization and injury of biological macromolecules were observed under a transmission electron microscope (TEM).The rate of ONPG hydrolysis and changes in activities of antioxidant enzymes were observed Raman spectroscopy.Results the changes of damaged cells involved morphological damage such as loss of the structural integrity of the wall and membrane,condensation of cellular material and leakage of significant amounts of cytoplasmic material,a more than four-fold increase of cell membrane permeabilization and damage to the structure of protein,nucleic acids and phospholipid.Conclusion UV disinfection can lead to a multi-target damage.