Effects of PM2.5 on Expression of Pigment Epithelium-derived Factor in Bronchial Epithelial Cells
10.3969/j.issn.1006-9771.2016.10.008
- VernacularTitle:PM2.5对支气管上皮细胞色素上皮衍生因子表达的影响
- Author:
Chenghua ZHU
;
Lijun SHI
;
Lingfeng ZHANG
;
Xiaolan JI
;
Qiang DU
- Publication Type:Journal Article
- Keywords:
particulate matter;
bronchial epithelial cells;
pigment epithelium-derived factor
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(10):1151-1153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of particulate matter (PM) 2.5 on the expression of pigment epithelium-derived factor (PEDF) protein in bronchial epithelial cells (BEAS-2B). Methods Human BEAS-2B were subcultivated, followed by low, medium and high concentrations of PM2.5 (25μg/ml, 50μg/ml, 100μg/ml) stimulation for 24 hours. The expression of PEDF protein in supernatant was ana-lyzed by enzyme-linked immunosorbent assay (ELISA), and the expression in BEAS-2B cells was detected by Western blotting. Results Compared with the control group, the expression of PEDF protein in supernatant and BEAS-2B cells induced by PM2.5 (25 μg/ml) in-creased, but no significance was found (t=-0.730, t=-1.840, P>0.05), and the expression induced by PM2.5 with the concentrations of 50μg/ml and 100μg/ml significantly increased (t>5.798, P<0.05). Conclusion PM2.5 with the concentrations of 50μg/ml and 100μg/ml could increase the expression of PEDF protein in a concentration-dependent manner both in supernatant and BEAS-2B cells.
