Influence of electroacupuncture on histomorphology of lacrimal glands and ocular surface in experimental dry eye syndrome
10.1007/s11726-016-0943-1
- VernacularTitle:电针对实验性干眼症泪腺和眼表组织形态学的影响
- Author:
Cuihong ZHANG
;
Lingling ZHANG
;
Dan ZHANG
;
Xiaoxu LIU
;
Yanting YANG
;
Zhiyuan LI
;
Ji ZHANG
;
Jue HONG
;
Jie LIU
;
Xiaopeng MA
;
Lingxiang WU
- Publication Type:Journal Article
- Keywords:
Acupuncture Therapy;
Electroacupuncture;
Dry Eye Syndrome;
Anatomy & Histology;
Rabbit
- From:
Journal of Acupuncture and Tuina Science
2016;14(5):320-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observethe influence of electroacupuncture (EA) on histomorphologies of lacrimal glands, cornea and conjunctiva in experimental dry eye syndrome, and to explore the repair effects of EA on lacrimal glands and ocular surface damage. Methods:Twenty-four healthy male New Zealand rabbits were randomly divided into a normal group, a model group, an EA group and a medication group, 6 rabbits in each group. Experimental dry eye syndrome models were prepared in rabbits by using 0.1% benzalkonium chloride for eye drops. Tear secretion volume, break-up time of tear film (BUT) and corneal fluorescein staining score were observed before and after the treatment. Periodic acid Schiff (PAS) staining method was used to observe the changes of conjunctival goblet cells in rabbits. After hematoxylin eosin (HE) staining, morphological changes of rabbit cornea, conjunctiva and lacrimal gland tissues were observed under light microscope. Results: Compared with the normal group, tear secretion volume and BUT were significantly reduced (bothP<0.01), while the corneal fluorescein staining score was significantly increased (P<0.01) in the model group. Compared with the model group, tear secretion volume and BUT were significantly increased, while the corneal fluorescein staining score was significantly decreased in the EA group and the medication group (allP<0.01). Compared with the normal group, the number of conjunctival goblet cells in the model group was significantly reduced; compared with the model group, the numbers of conjunctival goblet cells were all relatively higher in the EA group and the medication group. Pathological lesions of cornea, conjunctiva and lacrimal glands all showed improvement by HE staining in the EA group and the medication group after the intervention. Conclusion:EA can improve tear secretion and tear film stability of rabbit dry eye syndrome, and repair the pathologic lesions of conjunctival goblet cells, corneal epithelia, cornea, conjunctiva and lacrimal glands.
