Analysis the effect of different centrifugal conditions on the results of coagulation
10.3969/j.issn.1673-4130.2016.19.005
- VernacularTitle:离心条件对凝血三项检测结果的影响及分析
- Author:
Xuebin MA
;
Lu BAI
;
Qiangyuan ZHAO
- Publication Type:Journal Article
- Keywords:
coagulation testing;
centrifugal conditions;
platelet-poor plasma
- From:
International Journal of Laboratory Medicine
2016;37(19):2672-2674
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the effect of different centrifugal conditions on coagulation and ascertain the optimum centrifugal time for coagulation testing in the laboratory .Methods Navy General hospital check‐up and hospitalized patients were divided into three groups which were conventional group ,standard group and speed up the group respectively according the different centrifuga‐tion conditions .The platelet poor plasma (PPP) was harvested in the three different groups .The PT ,INR ,FIB and APT T were tested and the results were compared and analyzed .Samples with different platelets were centrifuged in normal condition or standard condition ,after that ,the residual platelet in plasma was detected .Results There was no significant difference of the PT ,INR ,FIB and APT T results among the routine group ,speed group and the standard group .The results of PPP was qualified 100% in samples which platelets were less than 500 × 109 /L with routine conditions and standards of centrifugal conditions .However ,the qualified result of PPP was dropped to 64% in those samples which platelets were higher than 500 × 109 /L with routine and standards of centrifugal conditions .Conclusion The conventional centrifugation conditions can meet clinical routine coagulation testing require‐ments ,the special types of coagulation assays or the samples which platelets count are more than 500 × 109 /L should centrifuge in accordance with the requirements of the CLSI centrifugal processing .
