The influence of high glucose on cytokine secreted by peritoneal mesothelial cells in rats and the intervention effect of matrine
10.3969/j.issn.1671-8348.2016.27.008
- VernacularTitle:高糖对大鼠腹膜间皮细胞分泌细胞因子的影响及苦参碱的干预作用
- Author:
Jianfang WU
- Publication Type:Journal Article
- Keywords:
glucose;
matrine;
tumor necrosis factor-α;
transforming grow th factor-β1;
peritoneal mesothelial cells
- From:
Chongqing Medicine
2016;45(27):3770-3772
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was designed to investigate the effect of matrine on high glucose‐induced tumor necrosis factor‐α(TNF‐α)and transforming growth factor‐β1 (TGF‐β1 ) overexpression in rat peritoneal mesothelial cells(PMCs) ,and to ex‐plore the possible mechanism in the peritoneal fibrosis .Methods ology The rat PMCs were incubated in vitro ,and then assigned in‐to 5 groups on the basis of the added drug concentrations :2 .5% glucose group (n=15 ,A);Glucose + 2 .5% matrine group (n=15 ,B);4 .25% glucose group (n=15 ,C);Glucose + 4 .25% matrine group (n=15 ,D);Blank control group (n=15 ,control group , added the DMEM/F12 medium) .The levels of TNF‐αand TGF‐β1 in the supernatants were measured by ELISA method at the dif‐ferent time (24h ,48h ,72h) ,and observed the changes of PMCs under the microscope .Results Compared with the groups of A ,C and D ,the PMCs in the control group and B group were small ,round ,spindle ,irregular or intensive .The expression of TNF‐αand TGF‐β1 in the A group was lightly higher than that of the control group ,but the level in the C group was significantly higher than that of the control group (P<0 .05) .As compared with the A group ,The level of TNF‐α and TGF‐β1 expressed in the B group were decreased (P> 0 .05 ) ,but the lever in the D group was significantly lower than that of the C group ( P< 0 .05 ) . Conclusion Matrine might be helpful to resist the overexpression of TNF‐αand TGF‐β1 induced by high glucose ,and then protect the PMCs .
