Influence of new baicalin-metal complexes on Kv1.4 and Cav3.2 ion channels
10.3867/j.issn.1000-3002.2016.09.005
- VernacularTitle:新型黄芩苷金属离子配合物对Kv1.4和Cav3.2离子通道的影响
- Author:
Ming GUO
;
Jun FAN
;
Yuan CHEN
;
Maoqing CHEN
;
Kai XU
;
Pingfeng BIAN
- Publication Type:Journal Article
- Keywords:
baicalin;
baicalin metal complexes;
ion channel;
whole-cell patch clamp technique
- From:
Chinese Journal of Pharmacology and Toxicology
2016;30(9):935-940
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of new baicalin(BC) metal ions(Co2+,Cu2+, and Ni2+)complexes(BMCs)on ion channels Kv1.4 and Cav3.2. METHODS HEK293 or CHO cells loaded with various ion channels(hERG,Kv1.2,Kv1.3,Kv1.4,Kv1.5,Kv1.6,Kv1.7,Kv1.8,Kir1.1, Kir2.1,KCNQ and Cav3.2)were obtained by stable transfection method. Whole-cell patch-clamp tech?nique was used to record current changes of each ion channel induced by BC and BMC in 10μmoL · L-1. The effect of different concentrations(0.3,1,3,10 and 30μmoL · L-1)of BC-Co and BC-Cu on Kv1.4 and Cav3.2 current was detected by whole-cell patch-clamp technique. RESULTS A model of HEK293 cells or CHO cells that stably expressed various ion channels was obtained. BMCs (BC-Co,BC-Cu and BC-Ni)had some impact on various ion channels,especially on Kv1.4 and Cav3.2. The inhibitory rate induced by BC-Co,BC-Cu and BC-Ni(10 μmol · L-1)was 91%,76% and-10%,respectively,for Kv1.4;and 43%,57%and-14%,respectively,for Cav3.2. IC50 of BC-Co was 1.69 and 0.81μmoL·L-1 for Kv1.4 and Cav3.2. IC50 of BC-Cu was 1.66 and 0.58μmoL · L-1 for Kv1.4 and Cav3.2. CONCLUSION BC-Cu and BC-Co concentration-dependently inhibit Kv1.4 and Cav3.2 ion channels.
