Chlorpyrifos exposure withdrawal induces delayed cytotoxicity in rat primary hippocampal neurons

VernacularTitle:氯吡硫磷染毒撤除对原代培养大鼠海马神经元细胞毒性的延迟效应
Author: Chunyan WU ; Changhui YAN ; Fenghua FU ; Yongbiao GUAN
Publication Type:Journal Article
Keywords: organophosphorus insecticides; chlorpyrifos; hippocampus; neurons,cultured
From: Chinese Journal of Pharmacology and Toxicology 2016;30(9):941-948
CountryChina
Language:Chinese
Abstract: OBJECTIVE To investigate the delayed cytotoxicity effect of chlorpyrifos (CPF) with?drawal on primary hippocampal neurons. METHODS Hippocampal neurons were prepared from SD rat fetuses on the 17th day of gestation. Seven days after culture,neurons were exposed to CPF 10 and 30 μmol · L-1,respectively,for 72 h or for 48 h followed by CPF withdrawal for 24 h. CCK-8 kit and neuronal nuclei(NeuN), 5-bromodeoxyuridine(BrdU) and β Ⅲ tubulin immunofluorescence expression methods were used to evaluate the cell viability. RESULTS Compared with normal control, no significant cytotoxicity was found after CPF 72 h continuous exposure. However,CPF 48 h expo?sure followed by CPF withdrawal for 24 h induced evident cytotoxicity. The amount of BrdU positive and β Ⅲ tubulin positive hippocampal neurons were both decreased significantly(P<0.05),and cell survival and viability reduced after CPF withdrawal. CONCLUSION CPF exposure withdrawal can induce more seriously delayed cytotoxicity than continuous exposure in rat primary hippocampal neurons.