Inhibitory effect of lead acetate on TRPA1 channel in mice and humans
10.3867/j.issn.1000-3002.2016.09.007
- VernacularTitle:醋酸铅对人和小鼠瞬时受体电位A1离子通道的抑制作用
- Author:
Hong ZHAO
;
Yuzhu SONG
- Publication Type:Journal Article
- Keywords:
lead acetate;
dorsal root ganglion;
neuron;
transient receptor potential A1 channel;
two-electrode voltage clamp;
calcium imaging
- From:
Chinese Journal of Pharmacology and Toxicology
2016;30(9):949-954
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the inhibitory effect of lead acetate on transient receptor potential A1(TRPA1)channel. METHODS TRPA1-mediated calcium influx in mice dorsal root ganglion(DRG) neurons and HEK293 cells expressing nouse TRP1 (mTRPA1) and human TRPA1 (hTRPA1) was recorded by intracellular calcium imaging. TRPA1-mediated currents were detected by two-electrode voltage clamp. RESULTS Lead acetate 3.0 and 10.0μmol·L-1 inhibited external calcium influx in DRG neurons by(36.7 ± 4.1)% and(79.4 ± 3.1)%(n=5),respectively. The inhibitory effect of lead acetate on hTRPA1-mediated current was concentration-dependent. Lead acetate 0.3, 1.0, 3.0, 10.0 and 30.0μmol · L-1 inhibited the amplitudes of currents by(1.0 ± 0.7)%,(11.6 ± 0.8)%,(57.7 ± 3.2)%,(93.6 ± 2.6)%and(91.2±2.0)%(n≥4),respectively,with the IC50 2.4μmol·L-1. CONCLUSION TRPA1 channel may be an endogenous target of lead. Lead acetate inhibits TRPA1 channel at a very low concentration.