Effects of luteolin on proliferation of osteosarcoma stem cells

VernacularTitle:木犀草素对骨肉瘤干细胞增殖的影响
Author: Naikun SUN ; Yaozong WANG ; Zhigang LIU ; Daijie FU ; Xifu SHANG ; Xu LI
Publication Type:Journal Article
Keywords: luteolin; osteosarcoma stem cells; proliferation; Ki67; JAK2/STAT3 signal pathway
From: Chinese Journal of Biochemical Pharmaceutics 2016;36(8):31-35
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of luteolin on the proliferation of osteosarcoma stem cells.Methods CD133 +osteosarcoma stem cells were separated from MG63 cells by flow cytometer.MTT was used to investigate the effects of luteolin(0,0.01,0.02,0.04 mg/mL)on the proliferation of osteosarcoma stem cells.Western blot was used to detect the levels of Ki67 protein and components of JAK2/STAT3 signal pathway in osteosarcoma stem cells induced.Results After sorting,the content of the CD133 +fraction was enriched up to(87.60 ±5.06)%.MTT assay showed that,compared with the control group,luteolin(0.01,0.02,0.04 mg/mL)inhibited proliferation of CD133 + osteosarcoma stem cells(P <0.05).Western blot also showed that luteolin significantly decreased the level of Ki67 compared with the control group(P<0.05).In addition,the luteolin inhibited the expression of p-JAK2 and p-STAT3 in JAK2/STAT3 signal pathway of CD133 + osteosarcoma stem cells compared with the control group ( P <0.05 ) . Conclusion Luteolin might be a suppressor of osteosarcoma stem cells.