Determination of Aliphatic Amines in Infant Food Packaging Materials by High Performance Liquid Chromatography
10.11895/j.issn.0253-3820.160277
- VernacularTitle:液相色谱法测定婴幼儿食品包装材料中的脂肪胺
- Author:
Cuncun SHENG
;
Shijuan ZHANG
;
Fangfang WANG
;
Zhongyin JI
;
Kun DOU
;
Jinmao YOU
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Gas purge-microsyringe extraction;
Packaging materials of baby food;
Fluorescence detection
- From:
Chinese Journal of Analytical Chemistry
2016;44(9):1365-1371
- CountryChina
- Language:Chinese
-
Abstract:
Aliphatic amines in infant food packaging materials were extracted and concentrated by 0 . 5 mL of acidified methanol using gas purge microsyringe extraction ( GP-MSE ) . Pre-column fluorescence labeling of amines was achieved in mild conditions with 10-ethyl-acridine-2-sulfonyl chloride ( EASC ) as labeling reagent. The derivatization was carried out at 60℃ and pH 10. The derivatives were successfully separated on a Hypersil GOLD column with excitation and emission wavelengths of 262 and 430 nm, respectively. The detection limits were in the range of 0. 4-0. 6 μg/kg, and the quantitation limits were in the range of 1. 2-2. 1 μg/kg. All analytes were in good linearity in the concentration range of 2. 0-2000 μg/L with correlation coefficients of higher than 0. 998. The developed method was characterized by celerity, accuracy and high sensitivity. It was successfully applied to the determination of aliphatic amines in infant food packaging materials.
