Research Progresses in Single Molecule Enzymology
10.11895/j.issn.0253-3820.160201
- VernacularTitle:单分子酶学研究进展
- Author:
Yan XU
;
Lele SUN
;
Yanjing GAO
;
Weiwei QIN
;
Tianhuan PENG
;
Di LI
- Publication Type:Journal Article
- Keywords:
Single molecule;
Enzyme;
Ribozyme;
Fluorescence resonance energy transfer;
Fluorescence microscopy;
Review
- From:
Chinese Journal of Analytical Chemistry
2016;44(9):1437-1446
- CountryChina
- Language:Chinese
-
Abstract:
The single molecule imaging and technologies that developed in 1990 s have successfully probed the dynamics of single molecule enzyme catalysis in real time in vitro. Ever since then, single molecule enzymology has entered the golden age of rapid developing. Individual features of each enzyme hidden in the overall average have been discovered, and many new catalytic mechanisms have been proposed. Single molecule enzymology sheds light on the dynamic interactions between enzymes and substrates or products, deepening the understanding of biochemical reactions. This review described the recent research progresses of single molecule protease and ribozyme.
