Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro
10.3969/j.issn.2095-4344.2016.37.002
- VernacularTitle:中药川芎对成骨细胞作用最佳浓度的体外实验
- Author:
Wei LU
;
Rui GUO
;
Gang ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(37):5483-5488
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation.
OBJECTIVE:To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I col agen expression.
METHODS:Mouse osteoblast cel line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cel counting kit-8 assay, and alkaline phosphatase activity and type I col agen level measured using ELISA method.
RESULTS AND CONCLUSION:Different concentrations of Ligustrazine al significantly promoted the osteoblast proliferation and type I col agen level compared with the blank control group (P<0.005). The activity of alkaline phosphatase in al Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P<0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I col agen expression, with the optimum concentration of 10 mg/L.
