Effects of joint immobilization on the repair of articular cartilage of the rat knee
10.3969/j.issn.2095-4344.2016.37.004
- VernacularTitle:关节制动对大鼠膝关节软骨缺损修复的影响
- Author:
Liyan XU
;
Jianxiong MA
;
Ying WANG
;
Lei SUN
;
Chunqiu ZHANG
;
Xinlong MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(37):5496-5503
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Joint immobilization is one of the methods used to treat joint pain and joint injury in the department of orthopedics. Compared with other treatment methods, immobilization can reduce the pain of the damaged synovial joints and avoid the contact stress and friction between the joints. However, immobilization can cause some serious complications such as joint contracture, osteoporosis and cartilage degeneration.
OBJECTIVE:To observe the effects of joint immobilization on the repair of cartilage injury of knee joint in rats.
METHODS:Osteochondral ful-thickness defects (2.5 mm in diameter;2 mm in depth) were created in the left femoral condyle fossa with a corneal trephine. 36 animals were randomly assigned into immobilization group and control group (n=18 per group). In the control group, animal models were established, without any treatment. In the immobilization group, after model establishment, rats were immobilized by a designed and modified simplified miniature Ilizarov fixator.
RESULTS AND CONCLUSION:(1) Repair rate of cartilage defect:No significant difference in repair rate was detected between immobilization group and control group. (2) Histological staining:Regeneration tissue was mainly fiber cel s in both groups. At 8 weeks after surgery, Wakitani score and Mankin score were higher in the immobilization group than in the control group (P<0.05). (3) Cartilage metabolic marker detection:Compared with the control group, at 8 weeks, C-telopeptide of type II col agen levels in the urine were significantly higher in the immobilization group than in the control group (P<0.05). (4) Results indicated that persistent immobilization could result in cartilage degeneration, and it was detrimental for cartilage repair.
