Simultaneous Contents Determination of Chloramphenicol and Diphenhydramine Hydrochloride in Chloro-benzene Alcohol Solution by HPLC
10.6039/j.issn.1001-0408.2016.27.34
- VernacularTitle:HPLC法同时测定氯苯醇溶液中氯霉素和盐酸苯海拉明的含量
- Author:
Shan LIU
;
Linlan JIANG
;
Xinmin XIE
;
Ming YAN
;
Dong LI
;
Tengxia LI
;
Hui LI
;
Lanlan LIN
;
Liping HAN
- Publication Type:Journal Article
- Keywords:
Chlorobenzene alcohol solution;
Chloramphenicol;
Diphenhydramine hydrochloride;
Content
- From:
China Pharmacy
2016;27(27):3847-3849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous contents determination of chloramphenicol and diphenhydr-amine hydrochloride in Chlorobenzene alcohol solution. METHODS:HPLC was performed on the column of Diamonsil C with mo-bile phase of phosphate buffer-acetonitrile(66.5∶33.5,V/V)containing 0.01 mol/L sodium heptane,column temperature was 35 ℃, flow rate was 1 ml/min,detection wavelength was 258 nm for diphenhydramine hydrochloride and 277 nm for chloramphenicol, the injection volume was 10μl. RESULTS:The linear range was 34.8-139.4μg/ml for chloramphenicol(r=0.999 8)and 93.0-930.5μg/ml for diphenhydramine hydrochloride (r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.70%-104.02%(RSD=2.2%,n=9)and 96.72%-101.72%(RSD=1.6%,n=9). CONCLUSIONS:The method is specific,accurate with good precision,and suitable for the quality control of Chlorobenzene alcohol solution.
