Protective effects of piperine on alveolar bone and collagen in a periodontitis model
10.3969/j.issn.2095-4344.2016.40.016
- VernacularTitle:胡椒碱对牙周炎模型牙槽骨和胶原的保护
- Author:
Run GE
;
Lan YANG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Periodontitis;
Models,Animal
- From:
Chinese Journal of Tissue Engineering Research
2016;20(40):6034-6040
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Piperine in models of pancreatitis, gout, middle cerebral artery infarction has anti-inflammatory, antioxidant and immune regulatory effects, but its effects on periodontitis model are not clear.
OBJECTIVE:To observe the protective effect of piperine on bone absorption and degradation of col agen in experimental rat models of periodontitis.
METHODS:Rat models of periodontitis were established by ligaturing the dental cervix of rat mandibular first molar with 3-0 silk. On day 1 before model establishment, rats were intragastrical y administered piperine 50 and 100 mg/kg. There were healthy control group and model group. Related detection was performed 8 weeks after model establishment.
RESULTS AND CONCLUSION:(1) Results of quantitative CT analysis:compared with the healthy control group, the distance from the first molar enamelo-cemental junction to the alveolar ridge crest was significantly lower in the model group (P<0.05). The degree of alveolar damage was significantly improved in the 50 and 100 mg/kg piperine groups compared with the model group (P<0.05). (2) Related factor protein expression:compared with the model group, matrix metal oproteinase-8,-13 and interleukin-1βprotein expression was significantly decreased in the 100 mg/kg piperine group (P<0.05);matrix metal oproteinase-8 protein expression was significantly decreased in the 50 mg/kg piperine group (P<0.05). (3) Col agen fiber morphology:compared with the model group, col agen fibers arranged orderly and col agen fiber area significantly increased in the 50 and 100 mg/kg piperine groups (P<0.05). (4) Results confirmed that piperine could reduce the alveolar bone resorption, reduce the degradation of col agen fibers and protect the periodontal tissues in models of periodontitis. Its mechanism is associated with the inhibition of matrix metal oproteinase-8,-13 and interleukin-1βprotein expression.
