Simultaneous Determination of 4 Components in Xiaoer Ganmao Granule by HPLC
10.6039/j.issn.1001-0408.2016.27.30
- VernacularTitle:HPLC法同时测定小儿感冒颗粒中4种成分的含量
- Author:
Guobao CHEN
;
Yanping ZHENG
;
Guojun YAN
;
Guiping SONG
;
Jia LIU
;
Ting CAI
- Publication Type:Journal Article
- Keywords:
Xiaoer ganmao granules;
(R,S)-goitrin;
Chlorogenic acid;
Luteoloside;
Isochlorogenic acid A;
HPLC
- From:
China Pharmacy
2016;27(27):3836-3838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of(R,S)-goitrin,chlorogenic acid,luteolo-side and isochlorogenic acid A in Xiaoer ganmao granule. METHODS:HPLC was performed on the column of Hedera C18 with mo-bile phase of acetonitrile-0.1% formic acid aqueous at a flow rate of 1.0 ml/min,the detection wavelength was 254 nm,330 nm and 370 nm,column temperature was 40 ℃,and the injection volume was 5 μl,RESULTS:The linear range was 6.6-105 μg/ml for (R,S)-goitrin (r=0.999 9),9-140 μg/ml for chlorogenic acid (r=0.999 9),9-144 μg/ml for luteoloside (r=0.999 8) and 9-138 μg/ml for isochlorogenic acid A(r=0.999 6);the limits of quantitation were 330 ng,450 ng,450 ng and 450 ng,limits of detection were 66 ng,90 ng,90 ng and 90 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 95.01%-98.77%(RSD=1.48%,n=6),95.14%-98.91%(RSD=1.52%,n=6),95.11%-97.54%(RSD=0.93%,n=6) and 95.58%-99.63%(RSD=1.73%,n=6). CONCLUSIONS:The method is simple and accurate,and suitable for the simultaneous determination of(R,S)-goitrin,chlorogenic acid,luteoloside and isochlorogenic acid A in Xiaoer ganmao granule.
