Analysis of Volatile Components in Cladia aggregata by SPME-GC-MS
10.6039/j.issn.1001-0408.2016.27.32
- VernacularTitle:SPME-GC-MS法分析聚筛蕊挥发性化学成分
- Author:
Chao ZHAO
;
Guoying HAN
;
Hongmei LI
;
Xin ZHOU
- Publication Type:Journal Article
- Keywords:
Cladia aggregate;
Lichens;
SPME;
GC-MS
- From:
China Pharmacy
2016;27(27):3843-3844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the volatile components of Cladia aggregate by SPME-GC-MS. METHODS:The column was ZB-5 MSI 5% benzene-95% dimethylpolysiloxane fused silica capillary column,programmed temperature was heated from 5 ℃/min to 220 ℃(maintaining 2 min),detector was flame ionization detector,carrier gas was high purity He(99.999%),the column flow rate was 1.0 ml/min,vaporization room temperature was 250 ℃,splitless injection. MS conditions:ion source was electron ionization source,ionizing voltage was 70 eV, interface temperature was 280 ℃,ion source temperature was 230 ℃, quadrupole temperature was 150℃,emission current was 34.6μA,multiplier voltage was 1 502 V,solvent delay time was 1.5 min, and quality range was 30-500 amu. RESULTS:19 volatile chemical components were separated and identified from C. aggregate, mainly olefins an aldehydes,alcohols,ketones,furans oxygenated volatile chemical components,relative contents accounted for 63.55% to all chromatographic peaks in total ion chromatogram,in which,2,4-octadiene(29.66%),hexanal(5.93%),3-meth-yl-1,4-heptadiene(5.45%)and furan(4.65%)showed the highest contents. CONCLUSIONS:The study has basically made clear the main volatile components in C. aggregate,which provide certain scientific basis for further exploring C. aggregate.
