Simultaneous Determination of Chlorogenic Acid,Hesperidin and Baicalin in Shubiaoling Granule by HPLC

VernacularTitle:HPLC法同时测定疏表灵颗粒中绿原酸、橙皮苷、黄芩苷的含量
Author: Ang LI ; Hongyu KAN ; Nan ZHANG
Publication Type:Journal Article
Keywords: Shubiaoling granule; Chlorogenic acid; Hesperidin; Baicalin; HPLC
From: China Pharmacy 2016;27(27):3872-3874
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,hesperidin and baicalin in Shubiaoling granule. METHODS:HPLC was performed on the column of SAGA Tri-Sal C18 with mobile phase of acetonitrile-0.5%phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 320 nm,column temperature was 30℃,in-jection volume was 10μl. RESULTS:The linear range was 2.463-240.5μg/ml for chlorogenic acid(r=0.999 9),6.577-642.3μg/ml for hesperidin(r=0.999 8)and 1.708-166.8 μg/ml for baicalin(r=0.999 8);RSDs of precision,stability and reproducibility tests were no more than 1.8%;recoveries were 97.02%-102.40%(RSD=1.9%,n=6),96.55%-100.20%(RSD=1.6%,n=6) and 97.51%-100.70%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple,accurate with high sensitivity and good reprosucibility,and can be used for the simultaneous determination of chlorogenic acid,hesperidin and baicalin in Shubiaoling granule.