Content Determination of L(-)-Anabasine in Different Medicinal Parts of Alangium chinense by HPLC
10.6039/j.issn.1001-0408.2016.27.45
- VernacularTitle:HPLC法测定八角枫药材不同药用部位中L（-）-八角枫碱的含量
- Author:
Qiyong WANG
;
Yaling XU
- Publication Type:Journal Article
- Keywords:
HPLC;
Alangium chinense;
L(-)-Anabasine;
Different medicinal parts
- From:
China Pharmacy
2016;27(27):3877-3879
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of L(-)-Anabasine in different medicinal parts (stem,rhizome,fibrous root,rootlet) of Alangium chinense,and provide reference for its development and utilization. METH-ODS:HPLC was performed on the column of Thermo C18 with mobile phase of methanol-phosphate buffer solution(22∶78,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 259 nm,column temperature was 30 ℃;the injection volume was 10 μl. RESULTS:The linear range of L(-)-Anabasine was 0.020 62-0.257 80μg(r=0. 999 9);the limit of quantitation was 1.7 ng,limit of detection was 0.5 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.38%-98.86%(RSD=0.6%,n=6). The content of L(-)-Anabasine in different medicinal parts was the fibrous root>the rootlet>the rhizome>the stem. CONCLUSIONS:The method is simple,accurate,reproducible and specific,and suitable for the content determination of L(-)-Anabasine in different medicinal parts(stem,rhizome,fibrous root,rootlet)of A. Chinense.
