Regulatory function of microRNA-182 in apoptosis of nucleus pulposus cells
10.3969/j.issn.2095-4344.2016.42.010
- VernacularTitle:髓核细胞凋亡调控中的MicroRNA-182
- Author:
Xiaowen QIU
;
Xijing HE
;
Dong WANG
;
Haopeng LI
;
Bo ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(42):6296-6301
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous study has found that hsa-miR-182 is probably related to the apoptosis-related genes such as cytochrome C (Cycs C) and calcineurin subunit CnB (PPP3R1) in nucleus pulposus cells.
OBJECTIVE:To determine whether miR-182 plays a regulatory role in nucleus pulposus cel apoptosis by detecting the relative gene expression levels after transfecting miR-182 with Cycs C and PPP3R1 into nucleus pulposus cel s via plasmid delivery.
METHODS:After a bioinformatics prediction about miR-182, miR-182 and target genes were transfected into the nucleus pulposus cel s, and at the same time, blank control group was established. Then the expression levels of the target genes were detected through cel lysis.
RESULTS AND CONCLUSION:miR-182 significantly inhibited the expression of Cycs C in nucleus pulposus cel s compared with the blank control group (P<0.05). Compared with the blank control group, miR-182 made no inhibitory effect on the expression of PPP3R1. These findings suggest that miR-182 may play a regulatory part in nucleus pulposus cel apoptosis by inhibiting the expression of Cycs C.
