Transplanting umbilical cord blood mesenchymal stem cells in ovarian cancer chemotherapy
10.3969/j.issn.2095-4344.2016.41.011
- VernacularTitle:脐血间充质干细胞移植在卵巢癌化疗损伤中的作用
- Author:
Xia LI
;
Donghui WANG
;
Liang GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(41):6151-6157
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Existing evidence has confirmed that umbilical cord blood mesenchymal stem cel s have an effect on functional recovery of a variety of damaged cel s.
OBJECTIVE:To explore the effect of umbilical cord blood mesenchymal stem cel transplantation on ovarian cancer chemotherapy.
METHODS:Sixty healthy adult female Wistar rats were randomly divided into normal control group, damage group and treatment group (n=20/group). There was no treatment in the control group, and a rat model of ovarian cancer chemotherapy damage was made in the damage group and treatment group. After successful modeling, rats in the control group were given normal saline injection via the tail vein, and those in the damage and treatment groups were given paclitaxel chemotherapy and pacligaxel chemotherapy plus umbilical cord blood mesenchymal stem cel transplantation, respectively. After transplantation of 2 weeks, mRNA and protein expressions of XAF1 and Survivin in ovarian tumor tissues were detected by RT-PCR and western blot assay, respectively. Apoptosis in ovarian cancer cel s were detected using TUNEL method.
RESULTS AND CONCLUSION:Compared with the damage group, a significant up-regulation of XAF1 mRNA and protein but a remarkable down-regulation of Survivin mRNA and protein were obtained in the treatment group (P<0.05). A severe damage to the ovarian tissues was visible in the damage group, presenting with large hemorrhage and necrosis area. This damage was markedly reduced in the treatment group. Additional y, the apoptotic rate of ovarian cancer cel s was significantly higher in the treatment group than the damage group (P<0.05). Al these findings indicate that umbilical cord blood mesenchymal stem cel transplantation aids in ovarian cancer chemotherapy to promote ovarian tissue repair in rats, and XAF1 and Survivin cannot be ignored in tumor angiogenesis and ovarian cancer cel apoptosis.
