Study on the Quality Standard for Fufang Ningshen Granule
10.6039/j.issn.1001-0408.2016.30.35
- VernacularTitle:复方宁神颗粒的质量标准研究
- Author:
Yang HUANG
;
Wenyi ZANG
;
Chunqin MAO
;
Tulin LU
;
Yaping ZHOU
- Publication Type:Journal Article
- Keywords:
Fufang ningshen granule;
TLC;
HPLC;
Spinosin;
Verbascoside;
Quality standard
- From:
China Pharmacy
2016;27(30):4285-4287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standards for Fufang ningshen granule. METHODS:TLC was used for the qualitative identification of Radix angelicae and White paenoy;HPLC was adopted for the contents determination of spinosin and verbascoside:the column was Kromasil C18 with the mobile phase of acetonitrile-0.1% acetic acid(16∶84,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temerpature was 30 ℃. RESULTS:The TLC spots of R. angelicae and W. pae-noy were clear with good separation,negative control without interference. The linear range was 0.055 44-0.277 2μg for spinosin(r=0.999 4)and 0.055 98-0.279 9 μg for verbascoside(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 95.62%-100.53%(RSD=1.77%,n=9) and 95.63%-102.57%(RSD=2.74%,n=9). CONCLU-SIONS:The standard can be used for the quality control of Fufang ningshen granule.
