Study on the Quality Standard of Solanum tuberdsm

VernacularTitle:紫肉甘薯药材的质量标准研究
Author: Luping WANG ; Wencheng WANG ; Rong TIAN ; Qixiao JIANG ; Chunbo WANG ; Zhongguo SUI
Publication Type:Journal Article
Keywords: Solanum tuberdsm; Oxidized cyaniding; TLC; HPLC; Quality standard
From: China Pharmacy 2016;27(30):4288-4290
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish the quality standard for Solanum tuberdsm. METHODS:The microscopic characteristics were detected;TLC was used for qualitative identification;moisture,ash,heavy metals and the amount of extract were deter-mined;HPLC was conducted for content determination of oxidized cyanidin:the column was Alltima C18 with mobile phase of ace-tonitrile-0.1% trifluoroacetic acid(80∶20,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 538 nm,column tempera-ture was 30℃. RESULTS:It showed clear microscopic identification map. S. tuberdsm TLC had clear spots and well separated. The water content was 8.5%-11.0%;total ash was 2.0%-2.9%;Pb was lower than 5 ppm and alcohol-soluble extracts was 15.6%-22.3%. The linear range of oxidized cyanidin was 0.043-0.215 μg(r=0.999 8);RSDs of precision,stability and reproduc-ibility tests were lower than 2.0%;recovery was 95.50%-99.65%(RSD=1.35%,n=9). CONCLUSIONS:The established method can be used for the quality control of S. tuberdsm.