Safety assessment of mammary gland stem cells from normal tissues in breast cancer patients
10.3969/j.issn.2095-4344.2016.41.007
- VernacularTitle:来源于乳腺癌正常组织乳腺干细胞的安全性
- Author:
Xiaojuan BI
;
Minggang FU
;
Liying GUO
;
Sha LIU
;
Yilamu DILIMINA
;
Chenming GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(41):6125-6130
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The cultivation of mammary gland stem cel s is of great significance for the development of mammary gland and breast cancer.
OBJECTIVE:To seek an easy method to isolate and culture mammary gland stem cel in vitro, and verify the safety of cel s.
METHODS:Mammary epithelial cel s were isolated from normal tissues surrounding breast cancer, and CD49f-and EPCAM-positive cel s were sorted using flow cytometry fol owed by surface marker analysis and cel colony formation ability analysis. Afterwards, real-time fluorescent quantitative PCR was used to detect C-erbB-2 and Maspin mRNA expression in mammary gland stem cel s, breast cancer tissues and normal tissues surrounding breast cancer.
RESULTS AND CONCLUSION:Human mammary gland stem cel s were successful y cultured and highly expressed CD49f and EPCAM, with the presence of mixed colony, pleural epithelial cel colony, and myoepithelial cel colony. c-erbB-2 was lowly expressed while Maspin highly expressed in mammary gland stem cel s. Our experimental findings indicate that the mammary gland stem cel s derived from normal tissue surrounding breast cancer have biological safety.
