Molecular Identification of Sparganium Stoloniferum and its Adulterants Based on ITS2 Sequence
- VernacularTitle:基于ITS2序列的三棱及其混伪品的分子鉴定
- Author:
Ying LUO
;
Xiaoxuan ZENG
;
Keli CHEN
;
Yimei LIU
- Publication Type:Journal Article
- Keywords:
Sparganium stoloniferum;
ITS2 sequence;
Adulterants;
Identification
- From:
China Pharmacist
2016;19(10):1813-1815
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore a new method to identify Sparganium stoloniferum and its adulterants by ITS2 regions. Methods:Eight samples of Sparganium stoloniferum and its adulterants were collected with five species, and 6 species with 23 ITS2 sequence of Sparganium stoloniferum and its adulterants were downloaded from Genbank. The intraspecific and interspecific K2P distances of Spar-ganium stoloniferum and its adulterants were calculated by MEGA5. 0, and the phylogenetic tree was constructed by MEGA 5. 0. Re-sults:The maximum intraspecific K2P distance of Sparganium stoloniferum was 0. 038,while the minimum interspecific K2P distance was 0. 697. The phylogenetic tree showed that Plantago asiatica was different obviously from its adulterants. The different samples of Sparganium stoloniferum were gathered together and could be distinguished from its adulterants by the NJ tree. Conclusion: ITS2 se-quence is able to identify Sparganium stoloniferum and its adulterants correctly, which provides a new method for the identification of Sparganium stoloniferum.
