Effect of transplantation of bone marrow mesenchymal stem cells on gastric cancer cell line SGC7901 in a rat model of gastric cancer
10.3969/j.issn.2095-4344.2016.41.012
- VernacularTitle:骨髓间充质干细胞移植对胃癌模型大鼠肿瘤细胞的影响
- Author:
Chao CHEN
;
Haitao LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(41):6158-6163
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone marrow mesenchymal stem cel s can migrate into tumor tissues, as reported in recent studies.
OBJECTIVE:To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cel s on proliferation and differentiation of gastric cancer cel line SGC7901 in rats.
METHODS:Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cel line SGC7901, and then, in cel transplantation group, each rat underwent an intraperitoneal injection of 1×107 bone marrow mesenchymal stem cel s. After transplantation, the targeting ability of bone marrow mesenchymal stem cel s was detected using fluorescent DiI labeling. Cyclin D2 mRNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cel s was observed by in situ terminal labeling method.
RESULTS AND METHODS:Bone marrow mesenchymal stem cel s successful y migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cel transplantation group were significantly higher than those in the model group (P<0.05). It was observed that the number of apoptotic cancer cel s was significantly reduced in the cel transplantation group compared with the model group (P<0.05). These findings indicate that bone marrow mesenchymal stem cel s have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cel s.
