Adipose-derived mesenchymal stem cell transplantation for type 1 diabetes in a rat model
10.3969/j.issn.2095-4344.2016.41.014
- VernacularTitle:脂肪间充质干细胞移植治疗1型糖尿病模型大鼠的作用
- Author:
Xiaohui LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(41):6171-6176
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Stem cel transplantation is a promising treatment for type 1 diabetes mel itus. Adipose-derived mesenchymal stem cel s have become another hotspot fol owing bone marrow mesenchymal stem cel s.
OBJECTIVE:To investigate the therapeutic effect of adipose-derived mesenchymal stem cel transplantation on type 1 diabetes in a rat model.
METHODS:Forty-five Sprague-Dawley rats were randomized into normal, model and cel transplantation. Animal model of type 1 diabetes was made in the latter two groups through intraperitoneal injection of streptozotocin. Seven days after modeling, rats in the three groups were given intraperitoneal injection of normal saline, serum-free DMEM or adipose-derived mesenchymal stem cel suspension, respectively. Two weeks after injection, body mass, blood glucose level, insulin secretion and PDX-1 mRNA in the pancreatic tissue of rats were monitored and detected in the three groups.
RESULTS AND CONCLUSION:After modeling, the body mass of rats were lowered, and increased gradual y in the cel transplantation group at 2 weeks after cel transplantation, but it was stil decreased in the model group. Compared with the normal group, the fasting blood glucose level was significantly higher in the model group (P<0.05), but it was reduced significantly after cel transplantation (P<0.05). Compared with the normal group, the insulin level was reduced significantly in the model group (P<0.05), but it was increased significantly after cel transplantation (P<0.05). Highest and lowest PDX-1 mRNA expressions were obtained in the normal and model groups, respectively;and there was a significant difference between groups (P<0.05). Al these findings show that adipose-derived mesenchymal stem cel transplantation relieves hyperglycemia in rats by promoting the expression of PDX-1 in the rat pancreatic tissue.
