Adipose-derived mesenchymal stem cell transplantation for treatment of chronic obstructive pulmonary disease
10.3969/j.issn.2095-4344.2016.41.015
- VernacularTitle:脂肪间充质干细胞移植治疗慢性阻塞性肺疾病
- Author:
Shuan HAO
;
Qi WU
;
Maojun WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(41):6177-6182
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Chronic obstructive pulmonary disease is progressive respiratory disease characterized by airflow limitation.
OBJECTIVE:To investigate the therapeutic effect of adipose-derived mesenchymal stem cel transplantation in rats with chronic obstructive pulmonary disease.
METHODS:Sixty healthy male Sprague-Dawley rats were randomly divided into control, model and treatment groups (n=20 per group). Rat models of chronic obstructive pulmonary disease were made in the model and treatment group, while no treatment was done in the control group. After modeling, rats in the treatment group were given tail vein injection of CM-Dil-labeled adipose-derived mesenchymal stem cel s, and rats in the model and control groups given the same amount of normal saline. Rat pulmonary ventilation function, inflammatory factor level and pathological changes of the lung were detected at 14 days after cel transplantation.
RESULTS AND CONCLUSION:After modeling, reduced lung function was found in rats with chronic obstructive pulmonary disease, but the cel transplantation significantly improved this reduction (P<0.05). Compared with the model group, significantly reduced indexes were visible in the treatment group (P<0.05), including the total number of white blood cel s, the number of macrophages and neutrophils, levels of interleukin-8, tumor necrosis factorαand C-reactive protein in the bronchoalveolar lavage fluid as wel as serum level of malondialdehyde, while serum superoxide dismutase activity was increased significantly in the treatment group (P<0.05). In the treatment group, emphysema-like changes were mitigated and CM-Dil-labeled adipose-derived mesenchymal stem cel s were capable of homing to the lung tissue of rats with chronic obstructive pulmonary disease and survived. Al these findings show that adipose-derived mesenchymal stem cel transplantation can improve lung function of rats with chronic obstructive pulmonary disease, and reduce inflammatory response, which has for certain some therapeutic effects.
