Study on HPLC Fingerprint of Cassia Leschenaultiana from Different Regions
- VernacularTitle:不同产地短叶决明HPLC指纹图谱的研究
- Author:
Jiandong LIU
;
Huifang XU
;
Zhongqiang HUANG
- Publication Type:Journal Article
- Keywords:
Cassia leschenaultiana;
HPLC;
Fingerprint;
Mutual mode
- From:
China Pharmacist
2016;19(10):1869-1871
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint of Cassia leschenaultiana from different regions. Methods: The column was SinoChrom ODS-BP (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 1% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 285 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results: The fingerprint consisted of 13 common peaks. The range of similarity for ten batches of Cassia le-schenaultiana was 0. 839-0. 998. And the reference fingerprint of Cassia leschenaultiana was established by HPLC. Conclusion: The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.
