Determination of Carfilzomib for Injection by HPLC
- VernacularTitle:HPLC法测定注射用卡非佐米的含量
- Author:
Na LIU
;
Yanjiao WANG
;
Haixia LI
;
Wenmin GUO
- Publication Type:Journal Article
- Keywords:
HPLC;
Carfilzomib for Injection;
Determination
- From:
China Pharmacist
2016;19(10):1959-1960
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method to determine the content of carfilzomib for injection. Methods:The chroma-tographic column was a Waters symmetry C18(250 mm ×4.6 mm, 5 μm)column, the sample solvent was methanol, the mobile phase was 0. 05% trifluoroacetic acid-acetonitrile(57 ∶43) at a flow rate of 1. 0 ml · min-1 , the detection wavelength was 210 nm, the col-umn temperature was 25℃ and the injection volume was 10 ml. Results:The linear range of carfilzomib was 120-600 μg·ml-1 ( r=0. 999 7). The average recovery was 100. 4%( RSD=0. 24%,n=9). Conclusion:The method is rapid and effective, which can be used for the content determination of carfilzomib for injection.
