Determination of Triptolide, Total Diterpenoids and Total Alkaloids in Leigongteng Oral Solution
- VernacularTitle:雷公藤口服液中雷公藤甲素、总二萜内酯和总生物碱的含量测定
- Author:
Lina YANG
;
Qingfen WANG
;
Xiaoxia NI
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
Leigongteng oral solution;
Triptolide;
Total diterpenoids;
Total alkaloids;
Determination
- From:
China Pharmacist
2016;19(10):1986-1989
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the content determination of triptolide, total diterpenoids and total alkaloids in Leigongteng oral solution to provide basis for the quality control. Methods:An HPLC analysis was used to detect the content of triptol-ide, which was carried out on an Eclipse XDB-C18 column (150 mm × 4. 6 mm, 5μm) with the mobile phase consisting of acetonitrile-water with gradient elution. The flow rate was maintained at 1. 0 ml·min-1 , the column temperature was kept at 40℃ and the detec-tion wavelength was set at 218 nm. Using triptolide and wilforine as the contrast, the total diterpenoids and total alkaloids were deter-mined by UV-Vis spectrophotometry. Results:There were good linear relationship in the determination of triptolide, total diterpenoids (caculated as triptolide) and total alkaloids (caculated as wilforine) (r≥0. 999 8), the average recovery were 91. 96%, 90. 56%, 99. 18%, and the RSD were less than 3%. Conclusion:The method is with good reproducibility and stability, which can be used for the quality control of Leigongteng oral solution.
