The protection by Propofol for injured blood-spinal cord barrier in spinal cord ischemia reperfusion in rabbits
10.3969/j.issn.1006-5725.2016.20.023
- VernacularTitle:丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障的保护作用
- Author:
Lijie XIE
;
Jinxiu HUANG
;
Ji HU
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Spinal cord ischemia;
Propofol;
Blood-spinal cord barrier
- From:
The Journal of Practical Medicine
2016;32(20):3364-3368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Propofol in blood spinal cord barrier (BSCB) disruption induced by spinal cord ischemia reperfusion injury (SCIRI). Methods 72 Japanese white rabbits were randomly assigned into 3 groups: sham-operation group (S); ischemia/reperfusion group (I/R) and Propofol treatment group (I/R + P). The Group S was separated the aorta without cross-clamping. SCIRI was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Propofol was intravenously infused at 10 minutes before aortic clamping and at onset of reperfusion in the Group I/R + P. The Group S and Group I/R were intravenously infused 0.9%sodium chloride. Hind-limb motor function was assessed using Tarlov criteria, and histological observation by histological examination. The permeability of the BSCB was examined using EB as vascular tracers. The expression of MMP-9, claudin-5 and NF-κB were assessed by Western blot, RT-PCR. Results Propofol minimized the neuromotor dysfunction and histopathological deficits and attenuated EB extravasation. In addition, Propofol suppressed SCIRI-induced increase of MMP-9 and NF-κB. Finally, Propofol reduced the loss of claudin-5. Conclusion Propofol stabilizes the BSCB integrity after SCIRI. This beneficial effect is partly mediated by inhibition of MMP-9 and preservation claudin-5 and relates to inhibiting the NF-κB signal pathway.
