Clarification Process Optimization of Total Flavonoids Extract from Geum Aleppicum with ZTC1 +1-ⅡType Clarifying Agent
- VernacularTitle:ZTC1+1-Ⅱ型澄清剂处理追风七总黄酮提取液的工艺优选
- Author:
Cheng CHEN
;
Lu BAI
;
Guoping LUO
;
Ming WEN
- Publication Type:Journal Article
- Keywords:
ZTC1+1-Ⅱ type clarifying agent;
Geum aleppicum;
Total flavonoids;
Clarification process
- From:
China Pharmacist
2016;19(10):1872-1875
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the clarification process of total flavonoids extract from Geum aleppicum with ZTC1+1-Ⅱtype clarifying agent. Methods:With the solid removal rate, retention rate of the total flavonoids and clearance rate of protein as the indi-ces, single factor tests were adopted to study the effects of extract concentration, amount of clarifying agent, reaction temperature and reaction time on the clarification results. Results:The optimal clarification process was as follows:the extract concentration was 0. 5 g ·ml-1 , the amount of clarifying agent was 4% component B and 2% component A, the reaction temperature was at 60℃, and the soaking time was 60 min. The retention rate of total flavonoids was 91. 32%, the clearance rate of protein was 35. 82%, and the solid removal rate was 8. 1%. Conclusion:ZTC1+1-Ⅱ type clarifying agent has good effect on the clarification of total flavonoids extract from Geum aleppicum with promising feasibility and stability.
