Effects of serum of patients undergoing isoflurane and sevoflurane anesthesia on invasion and migration potential of human lung adenocarcinoma cell line A549
10.3969/j.issn.1006-5725.2016.20.042
- VernacularTitle:异氟醚和七氟醚麻醉患者血清对人肺腺癌A549细胞侵袭和迁移能力的影响
- Author:
Feng XU
;
Qiong HUANG
;
Tao ZHANG
;
Chengxiang YANG
;
Hua LIANG
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Sevoflurane;
Lung cancer;
Metastasis;
Matrix metalloproteinase
- From:
The Journal of Practical Medicine
2016;32(20):3432-3434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of serum from patients receiving isoflurane and sevoflurane on the invasion and migration ability of human lung adenocarcinoma cell line A549. Methods Twenty ASAⅠorⅡ lung cancer patients aged 40 ~ 68 yr undergoing radical surgery were randomly divided into sevoflurane group (SEV group, n = 10) and isoflurane group (ISO group, n = 10). The concentration of sevoflurane or isoflurane maintained 1.5 MAC during anesthesia. Ten healthy volunteers were selected as control group. Serum was separated from blood sample taken at the end of surgery. A549 cells were randomly divided into sevoflurane group (group SEV, n = 10), isoflurane group (group ISO, n = 10) and control group (group C, n = 10). Cells of SEV group and ISO group were treated with 10% serum as respect to anesthetics for 24 hours. Cells of group C were treated with serum of control group. The invasion ability of cells was evaluated by Transwell assay. The migration ability of cells was determined by wound healing assay. The expressions of MMP-2 and MMP-9 in A549 cells were detected by ELISA. Results Compared with group C and ISO group,the number of invasive cells in group SEV was reduced significantly (P < 0.05). The levels of MMP-2 and MMP-9 in group SEV were significantly decreased compared with those of group C and ISO group (P<0.05). Conclusion The serum of patients receiving sevoflurane anesthesia can attenuate the metastatic ability of A549 cells through inhibiting the expression of MMP-2 and MMP-9.
