The effect of Rad001 and ATO on activation of the excessive autophagy and apoptotic pathways in SKOV3 ovarian cancer

VernacularTitle:三氧化二砷联合Rad001对卵巢癌细胞株SKOV3自噬和凋亡的影响
Author: Nan LIU ; Qitao HUANG ; Zhen YUE ; Xuan LI ; Guobing LIU ; Mei ZHONG
Publication Type:Journal Article
Keywords: Ovarian cancer; ATO; Rad001; Autophagy; Apoptosis
From: The Journal of Practical Medicine 2016;32(19):3168-3172
CountryChina
Language:Chinese
Abstract: Objective To investigate the synergistic anticancer effect of ATO combined with Rad001 on human ovarian cancer cells in vitro and the underlying mechanisms. Methods SKOV3 cells were treated with Rad001, ATO and a combination of Rad001 and ATO, respectively. Cell relative luminescence units, viability, and combination index were tested. The apoptosis was quantified by Flow cytometry. Autophagy and apoptosis associated proteins were measured with immunoblotting. Results Significant decrease in live cell number were observed in cells treated with combination of Rad001 and ATO , compared with single compound treatment (P <0.05). Moreover, a higher rates of autophagy as well as apoptosis were observed in the combination treatment compared with single compound treatment (P < 0.05). Conclusions combination of Rad001 and ATO can result in synergistic cytotoxicity through activation of the excessive autophagy and apoptotic pathway.