Synthesis of peptide of BALF4 gene of Epstein-Barr virus and application in the detection of nasopharyngeal carcinoma
10.3969/j.issn.1006-5725.2016.19.020
- VernacularTitle:EB病毒BALF4基因的短肽合成及在鼻咽癌检测中的应用
- Author:
Jianhua HUANG
;
Yuan LIAO
;
Jiangling CHEN
;
Bo HU
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma;
Epstein-Barr Virus;
gp125;
BALF4
- From:
The Journal of Practical Medicine
2016;32(19):3187-3189
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the application of recombinant gp125 proteins in serological diagnosis of nasopharyngeal carcinoma (NPC). Methods B cell epitopes of EBV gp125 protein were forecasted by internet server and the peptides were synthesized and coated to microplate for the detection of EBV-IgA antibody in NPC patients and healthy donors by ELISA. Results Six peptides were synthesized, wherein two showed low specificity and others displayed significantly higher positive rate in the NPC group than in the healthy group (P <0.001). Conclusion The BALF4 gene peptides are successfully synthesized and epitopes with high specificity and strong immunogenicity are screened for the preparation of gp125 antigen with high specificity and sensitivity.
